Diminished inhibition of mitochondrial electron transfer from succinate to cytochrome c by thenoyltrifluoroacetone induced by antimycin.

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Diminished inhibition of mitochondrial electron transfer from succinate to cytochrome c by thenoyltrifluoroacetone induced by antimycin.

Thenoyltrifluoroacetone inhibits succinate-cytochrome c reductase activity of resolved succinate*cytochrome c reductase complex. Dixon plots of [succinate-cytochrome c reductase activity]-’ or [succinateubiquinone reductase activity]-’ versus concentration of inhibitor are consistent with there being a single site of inhibition by thenoyltrifluoroacetone in resolved reductase complex. This agre...

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The indispensability of phospholipid and ubiquinone in mitochondrial electron transfer from succinate to cytochrome c.

The indispensability of phospholipid and ubiquinone (Q) in mitochondrial electron transfer was studied by depleting phospholipid and Q in succinate-cytochrome c reductase and then replenishing the depleted enzyme. More than 90% of phospholipid and Q was removed by repeated ammonium sulfate-cholate fractionation. The depleted succinate-cytochrome c reductase showed no enzymatic activity for succ...

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Superoxide production and electron transport in mitochondrial oxidation of dihydroorotic acid.

Production of superoxide radical during oxidation of dihydroorotate in rat liver mitochondria was not affected by antimycin A, thenoyltrifluoroacetone, or added ubiquinone but was inhibited by orotate, a product inhibitor of dihydroorotate dehydrogenase. It appears likely that superoxide is generated at the primary dehydrogenase. Dihydroorotate dehydrogenase differs from succinate dehydrogenase...

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Introduction: Cognitive dysfunction is the most common problem of patients with Alzheimer disease (AD). The pathological mechanism of cognitive impairment in AD may contribute to neuronal loss, synaptic dysfunction, and alteration in neurotransmitters receptors. Mitochondrial synapses dysfunction due to the accumulation of amyloid beta (Aβ) is one of the earliest pathological features of AD. Th...

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Stoichiometry of H+ translocation coupled to electron flow from succinate to cytochrome c in mitochondria.

Measurements are reported of the number of H+ translocated from the matrix to the suspending medium as a pair of electrons passes through energy-conserving Site 2, as reflected by electron flow from succinate to the artificial electron acceptor ferricyanide in intact rat liver mitochondria. The reduction of ferricyanide was measured by dual wavelength spectrophotometry and the translocation of ...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1979

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)30054-6